Genotyping Analysis of Garlic Cultivars Grown in Egypt Using Simple Sequence Repeat (SSR) Markers with Post-Labeling Method

The main objective of this study is to compare the productive performance and genetic relationships among three garlic genotypes which are common in Egyptian market. Cultivars with fewer cloves gave higher yields. Eggaseed-1 cv. and Sids-40 cv. produced between 9.7-11.9 ton/fed (fresh yield) and Egyptian cv. gave between 8.8-9.1 ton/fed (fresh yield) in both seasons of experiment. Simple sequence repeat (SSR) markers with post-labeling method have been used to reveal the genetic diversity among garlic cultivars. Ten SSR markers which showed high polymorphic content were tested for genetic characterization in 18 garlic selections. A total of 61 DNA fragments were amplified by the 10 markers with an average of about 6.1 fragments per marker. The genetic distances for SSR data using 18 garlic selections were done and relationships among selections were portrayed graphically in the form of a dendrogram. The value of genetic similarity ranging from 65.77 to 91.54% was observed among the garlic cultivars. The highest genetic similarity of 91.54% was among Eggaseed-1 and Sids-40 selections while, the highest dissimilarity was seen between Egyptian and Eggaseed-1 selections. These results allow identifying highly related garlic colored garlic; Eggaseed-1 cv. and Sids-40 cv. and separate them from Egyptian cv. that is characterized as white garlic. From the current study, it may be concluded that SSR markers with post-labeling method are efficient tool for investigation of genetic relationship in garlic therefore, this can help for selection in the breeding program. [Haitham E. M. Zaki. Genotyping Analysis of Garlic Cultivars Grown in Egypt Using Simple Sequence Repeat (SSR) Markers with Post-Labeling Method. Life Sci J 2014;11(10):1341-1348]. (ISSN:1097-8135). http://www.lifesciencesite.com. 196. doi:10.7537/marslsj111014.196.